Clinical Diagnostics

The Easy-plex robot automates Easy-Plex reactions making it possible for all operators to run multiplex PCR assays.

Ideally suited to the clinical environment, the robot is designed to eliminate errors and avoid contamination. Select the desired assay in the setup wizard, enter the sample IDs and press Go!

All IVD panels are designed to operate in the Easy-Plex robot and are supplied with colour-coded reagent tubes for easy set-up.

Most of our bacterial assays do not need extraction giving a considerable saving.

Automated multiplex PCR

Setting up any assay is as simple as this: Quick-start checklist

All the assay specific protocols are contained in an electronic template selected in the Setup Wizard.

More details of operation are given in the product inserts and manual.

Manual

Custom assays

AusDiagnostics will design, evaluate and set up MT-PCR assays for you. Multiplexes from 12 targets to 72 targets are possible and can be loaded into 72 well Gene-Discs or 96 well plates. When using 72 well Gene-Discs you will be able to run the assay using our template on a Rotor-Gene 6000 and have the advantage of automated calling of your results.

To enter your custom design, you need to have the NCBI sequence ID's for your gene targets. You can also cut and paste a region of the target sequence that you would like to measure into the online form, or add a base range in the comments box. Note however that our software picks out the best place to put your primers for a successful assay so we recomment not restricting the algorithm when possible.

Assays already validated in our database are available at half price! Click here to start

AusDiagnostics

AusDiagnostics is an Austalian company serving the research and molecular pathology disciplines in the area of gene profiling. More...

Publications

Evaluation of Multiplex Tandem Real-Time PCR for Detection of Cryptosporidium spp., Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in Clinical Stool Samples.
Stark D, Al-Qassab SE, Barratt JL, Stanley K, Roberts T, Marriott D, Harkness J, Ellis JT.
J Clin Microbiol. 2011 Jan;49(1):257-62. Epub 2010 Nov 3.

Rapid Detection of Bordetella Pertussis in Clinical Specimens by Multiplexed-Tandem PCR.
Danny Ko, Lee Thomas, Keith Stanley, Tom Olma and Jon Iredell
Poster ASM 2010

Rapid Detection of Bacterial Faecal Pathogens Using Multiplexed-Tandem PCR.
Lee Thomas, Tom Olma and Jon Iredell
Poster ASM 2010

Multiplexed tandem polymerase chain reaction identifies strong expression of oestrogen receptor and Her-2 from single, formalin-fixed, paraffin-embedded breast cancer sections.
Thompson EW, Warton K, Blick T, Wafai R, Hill P, Stanley K.
Pathology. 2010 Feb;42(2):165-72.

Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness.
Elektra Szewczuk, Kiran Thapa, Terry Anninos, Kenneth McPhie, Geoff Higgins, Dominic E Dwyer, Keith K Stanley and Jonathan R Iredell
BMC Infectious Diseases 2010, 10:113.

Multiplexed tandem polymerase chain reaction identifies strong expression of oestrogen receptor and Her-2 from single, formalin-fixed, paraffin-embedded breast cancer sections.
Thompson EW, Warton K, Blick T, Wafai R, Hill P, Stanley K.
Pathology. 2010 Feb;42(2):165-72.

Comparison of whole blood, serum, and plasma for early detection of candidemia by multiplex-tandem PCR
Lau A, Halliday C, Chen SC, Playford EG, Stanley K, Sorrell TC
J Clin Microbiol. 2010 Mar;48(3):811-6. Epub 2009 Dec 30

Staurosporine augments EGF-mediated EMT in PMC42-LA cells through actin depolymerisation, focal contact size reduction and Snail1 induction - a model for cross-modulation.
Hugo HJ, Wafai R, Blick T, Thompson EW, Newgreen DF.
BMC Cancer. 2009 Jul 15;9:235

Colony Multiplex-Tandem PCR for Rapid, Accurate Identification of
Fungal Cultures
Anna Lau et al
JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2008, p. 4058–4060 Vol. 46, No.12

Comparison of a Commercial Multiplexed Tandem PCR (MT-PCR) System with Classical Virology Techniques for Screening Clinical Specimens for Respiratory Viruses
Sylvia Mai, Kevin Barratt, Lance Jennings,
Canterbury Health Laboratories, Christchurch New Zealand

Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens
Anna Lau et al
JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 2008, p. 3021-3027 Vol. 46, No. 9

Multiplexed Tandem PCR - Potential for Use in the Rapid Detection of MRSA in Clinical Samples
Lee Thomas et al
ASM 2008

Comparison of Real-time PCR Methods and Membrane Elisa for Rapid Detection of Group B Streptococcus
Tom Olma et al
ASM 2008  

MT-PCR: New Diagnostic Platforms for Invasive Fungal Infections,
Anna Lau
SYNTROPHY, Volume 9, Issue 7: 2008

Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection
Keith K. Stanley and Elektra Szewczuk
Nucleic Acids Research 2005 33(20)