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EC Certificate 003496 0007 Rev. 00
Development of a Multiplex Tandem PCR (MT-PCR) Assay for the Detection of Emerging SARS-CoV-2 Variants
Rapid genome sequencing in hospitals to identify potential vaccine-escape SARS-CoV-2 variants (Snell LB, Cliff PR, Charalampous T, Alcolea-Medina A, Ebie SART, Sehmi JK, Flaviani F, Batra R, Douthwaite ST, Edgeworth JD, Nebbia G. Lancet Infect Dis, 2021, doi: 10.1016/S1473-3099(21)00482-5)
Increased diagnostic yield of routine multiplex PCR compared to clinician requested testing for detection of Trichomonas vaginalis (Webb B, Crampton A, Francis MJ, Hamblin J, Korman TM, Graham M. Pathology, 2021, doi: 10.1016/j.pathol.2020.07.008)
Off-season RSV epidemics in Australia after easing of COVID-19 restrictions (Eden J, Sikazwe C, Xie R, Deng Y, Sullivan S, Michie A, Levy A, Cutmore E, Blyth C, Britton P, Crawford N, Dong X, Dwyer D, Edwards K, Horsburgh B, Foley D, Kennedy K, Minney-Smith C, Speers D, Smith D, Kok J, Barr I, Australian RSV Study Group. medRxiv, 2021, doi: 10.1101/2021.07.21.21260810)
Detection and identification of dermatophyte fungi in clinical samples using a commercial multiplex tandem PCR assay (Ross I, Weldhagen G, Kidd S. Pathology, 2020, doi: 0.1016/j.pathol.2020.03.002.)
Use of amplicon melt temperature to detect SARS-CoV-2 Lineage B.1.1.7 variant through the G28048T mutation in the ORF 8 gene (Hale R, Green A, Cunningham E, Paul J. Journal of Clinical Virology Plus, 2021, doi: 10.1016/j.jcvp.2021.100025.)
Multiplex RT-PCR provides improved diagnosis of skin and nail dermatophyte infections compared to microscopy and culture: a laboratory study and review of the literature (BB Lin, Pattle N, Kelley P, Jaksic AS. Diagnostic Microbiology and Infectious Disease, 2021, doi: 10.1016/j.diagmicrobio.2021.115413.)
Saliva as a non-invasive specimen for detection of SARS-CoV-2 (Williams E, Bond K, Zhang B, Putland M and Williamson DA. J. Clin. Microbiol. 2020. doi: 10.1128/JCM.00776-20.) (2020)
Rapid deployment of pathology services to a remote Australian quarantine setting during the COVID-19 pandemic (Branley J, O’Sullivan M, Polkinghorne A, Poljak M, Stephens D. Rapid deployment of pathology services to a remote Australian quarantine setting during the COVID-19 pandemic, Pathology. doi: 10.1016/j.pathol.2020.06.003.)
Respiratory syncytial virus hospitalisations among young children: a data linkage study (Prasad N, Newbern EC, Trenholme AA, Wood T, Thompson MG, Aminisani N, Huang QS, Grant CC. Epidemiology and Infection V147 e246. doi: 10.1017/S0950268819001377) (2019)
Evaluation of the AusDiagnostics MT CRE EU assay for the detection of carbapenemase genes and transferable colistin resistance determinants mcr-1/-2 in MDR Gram-negative bacteria (D Meunier, N Woodford, KL Hopkins. J Antimicrobial Chemotherapy 2018 Sept https://doi.org/10.1093/jac/dky347)
Quantitative multiplexed-tandem PCR for direct detection of bacteraemia in critically ill patients (Ginn AN, Hazelton B, Shoma S, Cullen M, Solano T, Iredell JR. Pathology. 2017 Apr;49(3):304-308. doi: 10.1016/j.pathol.2016.10.014. Epub 2017 Feb 24)
Genomics for molecular epidemiology and detecting transmission of carbapenemase-producing Enterobacterales in Victoria, Australia, 2012-2016 (Sherry NL, Lane CR, Kwong JC, Schultz M, Sait M1, Stevens K, Ballard S, Gonçalves da Silva A, Seemann T, Gorrie CL, Stinear TP, Williamson DA, Brett J, van Diemen A, Easton M, Howden BP. J Clin Microbiol. 2019 Jul 17. pii: JCM.00573-19. doi: 10.1128/JCM.00573-19)
Investigating the first outbreak of oriental theileriosis in cattle in South Australia using multiplexed tandem PCR (MT-PCR) (Gebrekidan H, Gasser RB, Perera PK, McGrath S, McGrath S, Stevenson MA, Jabbar A) (Ticks Tick Borne Dis. 2015 Jul;6(5):574-8. doi: 10.1016/j.ttbdis.2015.04.012. Ep)
Use of multiplexed tandem PCR to estimate the prevalence and intensity of Theileria orientalis infections in cattle (Perera PK, Gasser RB, Read E, Malmo J, Nguyen H, Nyein S, Cheng A, R Jex A, Rawlin G, Spithill TW, Jabbar A. Infect Genet Evol. 2015 Jun;32:68-73. doi: 10.1016/j.meegid.2015.02.021. Epub 20)
Evaluation of a Commercial Multiplex PCR for Rapid Detection of Multi Drug Resistant Gram Negative Infections (Chavada R, Maley M. Open Microbiol J. 2015 Aug 31;9:125-35. doi: 10.2174/1874285801509010125. eColle)
Comparison of the performance of three PCR assays for the detection and differentiation of Theileria orientalis genotypes (Perera PK, Gasser RB, Pulford DJ, Stevenson MA, Firestone SM, McFadden AM, Jabbar A) (Parasit Vectors. 2015 Mar 31;8:192. doi: 10.1186/s13071-015-0812-7)
Semiquantitative multiplexed tandem PCR for detection and differentiation of four Theileria orientalis genotypes in cattle (Perera PK, Gasser RB, Firestone SM, Smith L, Roeber F, Jabbar A. J Clin Microbiol. 2015 Jan;53(1):79-87. doi: 10.1128/JCM.02536-14. Epub 2014 Oct)
Prevalence of Shiga toxin-producing E.coli (STEC) in Tasmania, Australia (McCallum, C. MvGregor, A. and Vanniasinkam, T. Pathology 45, 681-688, 2013)
Evaluation of vancomycin resistance 3 multiplexed PCR assay for detection of vancomycin-resistant enterococci from rectal swabs (Cekin Y, Erman Daloğlu A, Oğn D, Ozhak Baysan B, Dağlar D, Inan D, Mutlu D, Ongt G, Colak D. Ann Lab Med. 2013 Sep;33(5):326-30. doi: 10.3343/alm.2013.33.5.326. Epub 2013 Au )
Rapid identification of Gram-positive pathogens and their resistance genes from positive blood culture broth using a multiplex tandem RT-PCR assay (Hazelton BJ, Thomas LC, Unver T, Iredell JR. J Med Microbiol. 2013 Feb;62(Pt 2):223-31. doi: 10.1099/jmm.0.050385-0. Epub 201)
Multiplex-tandem PCR for fungal diagnostics (Lau A, Stanley K, Sorrell T. Methods Mol Biol. 2013;968:195-201. doi: 10.1007/978-1-62703-257-5_15)
The impact of bacterial and viral co-infection in severe influenza (Blyth CC, Webb SA, Kok J, Dwyer DE, van Hal SJ, Foo H, Ginn AN, Kesson AM, Seppelt I, Iredell JR; ANZIC Influenza Investigators; COSI Microbiological Investigators) (Influenza and Other Respiratory Viruses. 2013, 7(2):168–76)
Comparison of four multiplex PCR assays for the detection of viral pathogens in respiratory specimens (Anderson TP, Werno AM, Barratt K, Mahagamasekera P, Jennings LC, Murdoch DR. J Virol Methods, 2013, 109-2) (2013)
Establishment of a robotic, high-throughput platform for the specific diagnosis of gastrointestinal nematode infections in sheep (Roeber F, Jex AR, Campbell AJ, Nielsen R, Anderson GA, Stanley KK, Gasser RB) (Int J Parasitol. 2012 Dec;42(13-14):1151-8. doi: 10.1016/j.ijpara.2012.10.005. E)
Development and validation of a novel molecular biomarker diagnostic test for the early detection of sepsis (Sutherland A, Thomas M, Brandon RA, Brandon RB, Lipman J, Tang B, McLean A, Pascoe R, Price G, Nguyen T, Stone G, Venter D. Crit Care. 2011 Jun 20;15(3):R149. doi: 10.1186/cc10274)
Musashi-1 expression in atherosclerotic arteries and its relevance to the origin of arterial smooth muscle cells: histopathological findings and speculations (Bobryshev YV, Tran D, Botelho NK, Lord RV, Orekhov AN) (Atherosclerosis. 2011 Apr;215(2):355-65. doi: 10.1016/j.atherosclerosis.2011.01)
Thermal Image Scanning for Influenza Border Screening: Results of an Airport Screening Study (Priest PC, Duncan AR, Jennings LC, Baker MG. PLoS One. 2011, 6(1):e14490)
Gene expression alterations in formalin-fixed, paraffin-embedded Barrett esophagus and esophageal adenocarcinoma tissues (Botelho NK, Schneiders FI, Lord SJ, Freeman AK, Tyagi S, Nancarrow DJ, Hayward NK, Whiteman DC, Lord RV. Cancer Biol Ther. 2010 Jul 15;10(2):172-9) (Epub 2010 Jul 26)
Multiplexed tandem polymerase chain reaction identifies strong expression of oestrogen receptor and Her-2 from single, formalin-fixed, paraffin-embedded breast cancer sections (Thompson EW, Warton K, Blick T, Wafai R, Hill P, Stanley K. Pathology. 2010 Feb;42(2):165-72)
Evaluation of Multiplex Tandem Real-Time PCR for Detection of Cryptosporidium spp., Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in Clinical Stool Samples (Stark D, Al-Qassab SE, Barratt JL, Stanley K, Roberts T, Marriott D, Harkness J, Ellis JT) (J Clin Microbiol. 2011 Jan;49(1):257-62. Epub 2010 Nov 3)
Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness (Szewczuk E, Thapa K, Anninos T, McPhie K, Higgins G, Dwyer DE, Stanley KK, Iredell JR. BMC Infectious Diseases, 2010, 10(113))
Comparison of a Rapid Antigen Test with Nucleic Acid Testing during Cocirculation of Pandemic Influenza A/H1N1 2009 and Seasonal Influenza A/H3N2 (Kok J, Blyth CC, Foo H, Patterson J, Taylor J, McPhie K, Ratnamohan VM, Iredell JR, Dwyer DE. Journal of Clinical Microbiology 2010, 48(1):290–1)
Enhanced Diagnosis of Pandemic (H1N1) 2009 Influenza Infection Using Molecular and Serological Testing in Intensive Care Unit Patients with Suspected Influenza (Iwasenko JM, Cretikos M, Paterson DL, Gibb R, Webb SA, Smith DW, Blyth CC, Dwyer DE, Shi JQ, Robertson P, and Rawlinson WD. Clinical Infectious Diseases 2010, 51(1):70–2)
Comparison of adult patients hospitalised with pandemic (H1N1) 2009 influenza and seasonal influenza during the “PROTECT” phase of the pandemic response (Chang YS, van Hal SJ, Spencer PM, Gosbell IB, and Collett PW. Medical Journal of Australia. 2010, 192(2):90-3)
Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness (Szewczuk E, Thapa K, Anninos T, McPhie K, Higgins G, Dwyer DE, Stanley KK, and Iredell JR. BMC Infectious Diseases, 2010, 10:113)
Comparison of whole blood, serum, and plasma for early detection of candidemia by multiplex-tandem PCR (Lau A, Halliday C, Chen SC, Playford EG, Stanley K, Sorrell TC. J Clin Microbiol. 2010 Mar;48(3):811-6. Epub 2009 Dec 30)
Staurosporine augments EGF-mediated EMT in PMC42-LA cells through actin depolymerisation, focal contact size reduction and Snail1 induction – a model for cross-modulation (Hugo HJ, Wafai R, Blick T, Thompson EW, Newgreen DF. BMC Cancer. 2009 Jul 15;9:235)
Influenza A testing and detection in patients admitted through emergency departments in Sydney during winter 2009: implications for rational testing (Jardine A, Conaty SJ, Cretikos MA, Su WY, Gosbell IB, van Hal SJ. Medical Journal of Australia 2010, 193(8): 455–9)
Outbreaks of Pandemic (H1N1) 2009 and Seasonal Influenza A (H3N2) On Cruise Ship (Ward KA, Armstrong P, McAnulty JM, Iwasenko JM, and Dwyer DE. Emerging Infectious Diseases. 2010, 16(11):1731-7)
Colony Multiplex-Tandem PCR for Rapid, Accurate Identification of Fungal Cultures (Lau A, Sorrell TC, Lee O, Stanley K, Halliday C. JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2008, p. 4058?4060 Vol. 46, No.1)
Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens (Lau A, Sorrell TC, Chen S, Stanley K, Iredell J, Halliday C. JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 2008, p. 3021-3027 Vol. 46, No. 9)
Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection (Keith K. Stanley and Elektra Szewczuk. Nucleic Acids Research) (2005)